New model of computer analysis for assessment of the effects of lesions induced by direct voltage current in isolated AV-node of rabbit

Introduction: The ablation technique is one of the important therapeutic interventions for treatment of AV Nodal tachyarrhythmia. Different animal models have been developed to study the effects of ablation on the functional interanodal structure. The aim of the present study was to develop a new model of computer analysis to produce lesions by using direct voltage in isolated perfused rabbit AV-Node. Methods: The model of Superfused-perfused isolated AV-Node of rabbit was used in our study. Posterior nodal extension (slow pathway) and anterior nodal approaches (fast pathway) were ablated by using direct voltage (100-110 V) in 30 sec. All stimulation protocols and computer analysis were performed by the custom made software that has been developed in the Electrophysiology laboratory of Golestan Cardiovascular Research Center. All protocols were applied to 2 groups (5 rabbits in each group). Results: His deflection was detected by software (AV-Node pack) and nodal recovery curved was constructed on line. By using specific electrophysiological criteria, we could precisely predict the place of nodal ablation. Slow pathway ablation caused significant ERP and AHmax prolongation and fast pathway ablation caused significant AHmin prolongation. Histology examination confirmed the ablation results. Conclusion: Computer analysis of nodal ablation is a new method to induce specific lesions in AV-nodal pathways. Changing dynamic electrophysiological behavior of AV-node after ablation is an important index for predication of outcome of ablations.